8ADD
Viral tegument-like DUBs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9747 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.010, 120.670, 34.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.370 - 1.900 |
| R-factor | 0.1655 |
| Rwork | 0.162 |
| R-free | 0.21070 |
| Structure solution method | SIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.848 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.20_4478) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.162 | 1.020 |
| Rmeas | 0.172 | |
| Number of reflections | 37456 | 2724 |
| <I/σ(I)> | 11.3 | 1.72 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10.6 | 5.3 |
| CC(1/2) | 0.998 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 20% w/v PEG 6000 |
