8AD6

Structure of DarB bound to c-di-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	105.617, 42.293, 64.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.810 - 1.520
Rwork	0.174
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yj8
RMSD bond length	0.006
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.810	1.570
High resolution limit [Å]	1.520	1.520
Rpim	0.021	0.251
Number of reflections	40317	998
<I/σ(I)>	19.2
Completeness [%]	88.0
Redundancy	7.3
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.16	0.2 M Lithium sulfate 0.1 M MES 6.0 20 % w/v PEG 4000