Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.270, 143.320, 104.370 |
| Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
| Resolution | 48.730 - 3.500 |
| R-factor | 0.2675 |
| Rwork | 0.266 |
| R-free | 0.28990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4P6V |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.730 | 48.730 | 3.700 |
| High resolution limit [Å] | 3.500 | 10.000 | 3.500 |
| Rmerge | 0.075 | 0.025 | 2.907 |
| Rmeas | 0.082 | 0.027 | 3.136 |
| Total number of observations | 225415 | ||
| Number of reflections | 32938 | 1448 | 4951 |
| <I/σ(I)> | 12.6 | 55.76 | 0.8 |
| Completeness [%] | 99.3 | 97.6 | 98.1 |
| Redundancy | 6.844 | 6.31 | 7.022 |
| CC(1/2) | 1.000 | 0.999 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 40 mM KSCN, 21.0% PEG 2000MME, 100 mM Tris-acetic acid, 8% 1-propanol |
