8AC9
Structure of Pseudomonas aeruginosa aminopeptidase, PaAP_T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.997, 52.502, 114.383 |
| Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
| Resolution | 48.045 - 2.351 |
| R-factor | 0.1848 |
| Rwork | 0.182 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ac7 |
| Data reduction software | xia2 |
| Data scaling software | xia2 (3.6.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.730 | 66.750 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.380 | 2.350 |
| Rmeas | 0.074 | 0.042 | 0.467 |
| Rpim | 0.028 | 0.017 | 0.204 |
| Total number of observations | 256358 | 13108 | 8870 |
| Number of reflections | 38179 | 2026 | 1795 |
| <I/σ(I)> | 19.2 | 49.8 | 2.6 |
| Completeness [%] | 99.6 | 100 | 92.6 |
| Redundancy | 6.7 | 6.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 70 % v/v MPD |
