8AC7
Structure of Pseudomonas aeruginosa aminopeptidase, PaAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.952, 85.953, 98.216 |
| Unit cell angles | 90.00, 93.56, 90.00 |
Refinement procedure
| Resolution | 64.627 - 1.400 |
| R-factor | 0.1567 |
| Rwork | 0.156 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hc6 5ib9 1tkj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.774 |
| Data reduction software | xia2 |
| Data scaling software | xia2 (3.6.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.950 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.057 | 0.660 |
| Rmeas | 0.061 | 0.736 |
| Rpim | 0.023 | 0.316 |
| Total number of observations | 1375192 | 45920 |
| Number of reflections | 203540 | 8909 |
| <I/σ(I)> | 20.6 | 1.1 |
| Completeness [%] | 99.4 | |
| Redundancy | 6.8 | 5.2 |
| CC(1/2) | 0.998 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate pH 4.5, 0.2 M zinc acetate, 10 % PEG3000, 20 % 2-propanol |
