8ABP

SUGAR-BINDING AND CRYSTALLOGRAPHIC STUDIES OF AN ARABINOSE-BINDING PROTEIN MUTANT (MET108LEU) WHICH EXHIBITS ENHANCED AFFINITY AND ALTERED SPECIFICITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	55.620, 71.930, 78.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.490
R-factor	0.175
RMSD bond length	0.020
RMSD bond angle	0.036
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	1.490 *
Rmerge	0.037 *
Total number of observations	118025 *
Number of reflections	45315 *
Completeness [%]	88.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG	10 (%)
3	1	drop	citrate	10 (mM)
4	1	drop		1 (mM)
5	1	drop	sodium azide	0.02 (%)
6	1	drop	sugar	10 (mM)	D-galactose, D-fucose, L-arabinose
7	1	reservoir	PEG	20 (%)