8AB4

Crystal structure of MreB from Geobacillus stearothermophilus ATCC7953 in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	46.140, 61.410, 50.920
Unit cell angles	90.00, 111.82, 90.00

Refinement procedure

Resolution	42.830 - 2.220
R-factor	0.2101
Rwork	0.208
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zpt
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.270	2.350
High resolution limit [Å]	2.220	2.220
Rmeas	0.090	1.610
Number of reflections	13182	2073
<I/σ(I)>	12.74
Completeness [%]	99.5	98.5
Redundancy	6.9
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	PEG 300, 2-(N-morpholino)ethanesulfonic acid