8AB2

Crystal Structure of the Lactate Dehydrogenase of Cyanobacterium Aponinum in its apo form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9840
Spacegroup name	I 41 2 2
Unit cell lengths	103.797, 103.797, 197.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.960 - 2.100
R-factor	0.185
Rwork	0.183
R-free	0.22340
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.190	2.176
High resolution limit [Å]	2.100	2.100
Rmerge	0.077
Rpim	0.026
Number of reflections	31611	2966
<I/σ(I)>	19.35
Completeness [%]	99.0	94.4
Redundancy	9.7
CC(1/2)	0.999	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	21 % Peg MME 550, 0.1 M NaCl, 0.1 M bicine pH 9