8AB1
Crystal structure of the PulL-PulM C-terminal domain heterocomplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 117.899, 117.899, 110.002 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 102.100 - 2.770 |
| R-factor | 0.27277 |
| Rwork | 0.272 |
| R-free | 0.29278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NMR models |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.426 |
| Data reduction software | AutoProcess |
| Data scaling software | AutoProcess |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.104 | 2.819 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.099 | 1.982 |
| Rmeas | 0.101 | 2.007 |
| Rpim | 0.016 | 0.311 |
| Number of reflections | 11982 | 586 |
| <I/σ(I)> | 25 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 38.8 | 41.1 |
| CC(1/2) | 0.999 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0%w/v PEG 3350, 0.2M KCl |
