8AAM

Crystal structure of MreB from Geobacillus stearothermophilus ATCC7953

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	46.670, 61.710, 51.010
Unit cell angles	90.00, 112.17, 90.00

Refinement procedure

Resolution	47.240 - 2.290
R-factor	0.2062
Rwork	0.202
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292122438
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.240	2.430
High resolution limit [Å]	2.290	2.290
Rmeas	0.110	1.490
Number of reflections	12172	1938
<I/σ(I)>	12.17
Completeness [%]	94.4
Redundancy	6.89
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	PEG 300, 2-(N-morpholino)ethanesulfonic acid