8A9X

Crystal structure of PulM C-ter domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	80.850, 135.529, 109.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.760 - 1.523
R-factor	0.2174
Rwork	0.217
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NMR structure
RMSD bond length	0.011
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.760	1.549
High resolution limit [Å]	1.520	1.520
Rmerge	0.070	0.951
Rmeas	0.073	0.987
Rpim	0.020	0.264
Number of reflections	91712	4530
<I/σ(I)>	17.6	2.2
Completeness [%]	100.0	100
Redundancy	13.5	13.7
CC(1/2)	0.999	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M HEPES pH 7.5, 0.2 M CaCl2