8A9M
Hippeastrum hybrid agglutinin, HHA, complex with beta-mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-04-30 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.8975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.370, 100.318, 62.177 |
| Unit cell angles | 90.00, 137.27, 90.00 |
Refinement procedure
| Resolution | 23.820 - 2.200 |
| R-factor | 0.1691 |
| Rwork | 0.167 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1msa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 23.820 | 23.810 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.133 | 0.098 | 0.389 |
| Rmeas | 0.148 | 0.116 | 0.436 |
| Rpim | 0.063 | 0.059 | 0.191 |
| Total number of observations | 848 | 6329 | |
| Number of reflections | 15296 | 204 | 1335 |
| <I/σ(I)> | 8.2 | 13.8 | 3.8 |
| Completeness [%] | 98.5 | 89.1 | 99.5 |
| Redundancy | 4.9 | 4.2 | 4.7 |
| CC(1/2) | 0.986 | 0.926 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50mM PBS, 2M Ammonium sulphate |
