8A8R

Crystal structure of TEAD4 in complex with YAP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	43.026, 76.710, 164.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.390 - 1.696
R-factor	0.224
Rwork	0.223
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	previous in-house structure
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.389	1.821
High resolution limit [Å]	1.696	1.696
Rmerge	0.066	0.622
Rmeas	0.072	0.694
Rpim	0.029	0.271
Number of reflections	39525	1977
<I/σ(I)>	13	2.5
Completeness [%]	64.5	17.1
Redundancy	6.3
CC(1/2)	0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM sodium acetate trihydrate pH 4.6 50 mM magnesium acetate tetrahydrate 25% PEG400