8A83

rsEGFP2 with a chlorinated chromophore in the fluorescent ON-state in a crystal dehydrated after illumination

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.19
Spacegroup name	P 21 21 21
Unit cell lengths	51.484, 58.980, 64.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.786 - 1.810
R-factor	0.2013
Rwork	0.200
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dty
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.790	1.875
High resolution limit [Å]	1.810	1.810
Number of reflections	18434	1806
<I/σ(I)>	16.16
Completeness [%]	99.5
Redundancy	2
CC(1/2)	1.000	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.76 M ammonium sulfate, 0.1 M HEPES; Cryoprotectant 1.6 M sucrose, 2.72 M ammonium sulfate, 0.16 M HEPES