8A6O

Room temperature rsEGFP2 with a chlorinated chromophore 600 fs after Photoexcitation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2021-06-19
Detector	MPCCD
Wavelength(s)	1.18
Spacegroup name	P 21 21 21
Unit cell lengths	51.990, 62.910, 72.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.455 - 1.630
R-factor	0.1719
Rwork	0.170
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pft
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.460	1.688
High resolution limit [Å]	1.630	1.630
Number of reflections	30206	2952
<I/σ(I)>	5.924
Completeness [%]	100.0
Redundancy	311.9
CC(1/2)	0.980	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	1.3M ammonium sulfate, 100mM Hepes pH 8.1, 20mM NaCl