8A6C
1 picosecond light activated crystal structure of bovine rhodopsin in Lipidic Cubic Phase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SwissFEL ARAMIS BEAMLINE ESA |
Synchrotron site | SwissFEL ARAMIS |
Beamline | ESA |
Temperature [K] | 294 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | PSI JUNGFRAU 1M |
Wavelength(s) | 1.38 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 61.510, 91.010, 151.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.990 - 1.800 |
R-factor | 0.3506 |
Rwork | 0.350 |
R-free | 0.39750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u19 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.678 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.100 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 79304 | 7852 |
<I/σ(I)> | 5.76 | 0.79 |
Completeness [%] | 100.0 | 100 |
Redundancy | 296.3 | 185.2 |
CC(1/2) | 0.974 | 0.622 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 9 | 294 | 36% PEG 600, 100 mM Bicine pH 9.0 |