8A5S

Crystal structure of light-activated DNA-binding protein EL222 from Erythrobacter litoralis crystallized in dark, measured illuminated.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-10
Detector	Bruker PHOTON II
Wavelength(s)	1.3418
Spacegroup name	P 21 21 21
Unit cell lengths	43.426, 51.852, 80.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.223 - 1.850
R-factor	0.1613
Rwork	0.161
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p7n
RMSD bond length	0.037
RMSD bond angle	1.667
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XSCALE
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.570	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.116	0.772
Rmeas	0.129	0.881
Rpim	0.055	0.418
Number of reflections	16065	955
<I/σ(I)>	10.9	1.9
Completeness [%]	99.7	99.9
Redundancy	5.2	4.2
CC(1/2)	0.996	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	21% (w/v) PEG 8000, 0.22 M MgCl2, 0.11 M MES pH 6.5