8A4O

Crystal structure of the Ustilago hordei effector protein Uvi2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97626
Spacegroup name	P 61 2 2
Unit cell lengths	69.050, 69.050, 232.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.760 - 1.350
R-factor	0.1665
Rwork	0.166
R-free	0.18110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8a14
RMSD bond length	0.024
RMSD bond angle	1.751
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.760	1.401
High resolution limit [Å]	1.350	1.350
Rmerge	0.121	4.728
Number of reflections	72525	6974
<I/σ(I)>	17.93	0.51
Completeness [%]	99.8
Redundancy	35.2
CC(1/2)	1.000	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M Na-Acetate pH 5.0, 10% MPD, pH 5.0