Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | H 3 |
| Unit cell lengths | 141.680, 141.680, 183.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.576 - 2.290 |
| Rwork | 0.178 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ztw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.318 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmeas | 0.110 | 1.117 |
| Number of reflections | 61983 | 4602 |
| <I/σ(I)> | 22.86 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.2 | |
| CC(1/2) | 1.000 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.7 M Ammonium sulphate, 0.05 M Sodium Cacodylate pH 6.5 |
