8A1I
Crystal structure of murine Armc8 isoform beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.635, 148.183, 67.140 |
| Unit cell angles | 90.00, 90.57, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.690 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.24840 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.701 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.168 | 2.921 |
| High resolution limit [Å] | 2.692 | 2.692 |
| Rpim | 0.085 | 0.739 |
| Number of reflections | 26256 | 1313 |
| <I/σ(I)> | 7.9 | 1.1 |
| Completeness [%] | 90.7 | 47.9 |
| Redundancy | 4.9 | 6 |
| CC(1/2) | 0.993 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 100 mM MES pH5.5 150 mM ammonium sulfate 8% (w/v) PEG 3350 |
