8A14

Crystal structure of the cerato-platanin-like protein Cpl1 from Ustilago maydis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97624
Spacegroup name	P 21 21 21
Unit cell lengths	85.250, 121.920, 181.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.840 - 1.900
R-factor	0.1505
Rwork	0.149
R-free	0.17660
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.333
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	1.960
High resolution limit [Å]	1.900	1.900
Rmerge	0.107	1.009
Number of reflections	148966	14665
<I/σ(I)>	18.24	2.6
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.8 M LiCl; 0.1 M Citrate pH 5.0; 22,5% PEG 6000