8A0X
Crystal structure of the HigB2-HigA2 tetramer in complex with operator DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.620, 113.620, 121.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.342 - 3.296 |
| R-factor | 0.2395 |
| Rwork | 0.238 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jaa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.342 | 3.490 |
| High resolution limit [Å] | 3.290 | 3.300 |
| Rmeas | 0.187 | 1.230 |
| Number of reflections | 12408 | 1921 |
| <I/σ(I)> | 12.3 | 2 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium iodide, 0.1 M MES pH 6.5, 25% PEG 4000 |
