8A0K
crystal structure of the kinetoplastid kinetochore protein Trypanosoma brucei KKT3 Divergent Polo-Box domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0072 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.998, 52.485, 102.152 |
| Unit cell angles | 84.11, 84.42, 73.25 |
Refinement procedure
| Resolution | 50.680 - 2.920 |
| R-factor | 0.2515 |
| Rwork | 0.250 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | partial Se-Met model |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.26) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.680 | 50.680 | 3.000 |
| High resolution limit [Å] | 2.920 | 13.060 | 2.920 |
| Rmerge | 0.155 | 0.106 | 1.022 |
| Rmeas | 0.184 | 0.125 | 1.213 |
| Rpim | 0.098 | 0.066 | 0.643 |
| Total number of observations | 65155 | 731 | 4756 |
| Number of reflections | 19067 | 212 | 1382 |
| <I/σ(I)> | 5.1 | 10.1 | 1.4 |
| Completeness [%] | 99.0 | 99.1 | 98.4 |
| Redundancy | 3.4 | 3.4 | 3.4 |
| CC(1/2) | 0.976 | 0.968 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 25% PEG3350, 0.1 M Bis-Tris pH 5.5 and 0.1 M tri-sodium acetate pH 4.5 |
