8A0A

Bacillus subtilis SPbeta prophage master regulator MrpR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	77
Detector technology	PIXEL
Collection date	2022-02-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976253
Spacegroup name	P 21 21 21
Unit cell lengths	49.550, 98.870, 162.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.450 - 2.900
R-factor	0.2175
Rwork	0.215
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold model of YopR
RMSD bond length	0.004
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	3.006
High resolution limit [Å]	2.900	2.900
Number of reflections	18329	1762
<I/σ(I)>	8.89	1.47
Completeness [%]	99.9	99.27
Redundancy	12.4	12.7
CC(1/2)	0.991	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium tartrate, 20% (w/v) PEG3350