7ZY4

Crystal structure of human CstF77 in complex with hFip1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	157.612, 157.612, 161.005
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.310 - 2.550
R-factor	0.2138
Rwork	0.212
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ooe
RMSD bond length	0.013
RMSD bond angle	1.353
Data reduction software	autoPROC (20211020)
Data scaling software	autoPROC (20211020)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.310	2.641
High resolution limit [Å]	2.550	2.550
Number of reflections	38981	3836
<I/σ(I)>	36
Completeness [%]	100.0	100
Redundancy	40.5	42.3
CC(1/2)	1.000	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278.15	0.1 M Bicine pH 9.0, 10% w/v PEG 20k, 2% v/v Dioxane