7ZX9

Streptavidin with a fluorescent substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999998
Spacegroup name	I 41 2 2
Unit cell lengths	57.710, 57.710, 183.617
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.024 - 1.546
Rwork	0.176
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pk2
RMSD bond length	0.010
RMSD bond angle	1.589
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.900	45.900	1.570
High resolution limit [Å]	1.546	8.470	1.550
Rmerge	0.092	0.049	2.466
Rmeas	0.095	0.051	2.643
Rpim	0.026	0.014	0.916
Number of reflections	22167	186	734
<I/σ(I)>	20.6
Completeness [%]	95.2
Redundancy	20	18.8	14.9
CC(1/2)	0.999	0.998	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	2.0 M ammonium sulfate, 0.1 M sodium acetate 26 mg/ml protein