7ZX4

Clathrin N-terminal domain in complex with a HURP phospho-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.87313
Spacegroup name	P 1 21 1
Unit cell lengths	48.774, 94.242, 88.981
Unit cell angles	90.00, 103.81, 90.00

Refinement procedure

Resolution	43.200 - 2.080
R-factor	0.2084
Rwork	0.206
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c9i
RMSD bond length	0.007
RMSD bond angle	1.440
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.099	0.390
Rmeas	0.123	0.475
Rpim	0.071	0.268
Number of reflections	45105	2302
<I/σ(I)>	6.9	1.3
Completeness [%]	96.0	97.7
Redundancy	2.7	2.7
CC(1/2)	0.909	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	50 mM Tris pH 7.5, 30 % PEG 6000