7ZVZ
Cytochrome c prime beta from Methylococcus capsulatus (Bath): Chemically Reduced Ferrous Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 105.560, 105.560, 105.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.350 - 1.680 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hih |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.322 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.090 | 43.090 | 1.720 |
| High resolution limit [Å] | 1.680 | 7.510 | 1.680 |
| Rmerge | 0.044 | 0.022 | 1.315 |
| Rmeas | 0.048 | 0.024 | 1.424 |
| Rpim | 0.018 | 0.009 | 0.541 |
| Total number of observations | 299156 | 3562 | 22468 |
| Number of reflections | 44860 | 563 | 3298 |
| <I/σ(I)> | 24.2 | 101.1 | 1.5 |
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 6.7 | 6.3 | 6.8 |
| CC(1/2) | 1.000 | 0.999 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 15 mg/ml protein mixed with 10 mM ZnSO4, 35% PEG 550, 100 mM MES pH 6.5 |
