7ZV2

HUMAN PRMT5:MEP50 Crystal Structure With MTA and Fragment Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9198
Spacegroup name	I 2 2 2
Unit cell lengths	104.526, 138.844, 179.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.710 - 2.010
R-factor	0.2345
Rwork	0.232
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eml
RMSD bond length	0.007
RMSD bond angle	1.455
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.710	2.150
High resolution limit [Å]	2.010	2.010
Number of reflections	46129	2455
<I/σ(I)>	13.2
Completeness [%]	93.4	81.9
Redundancy	11.7
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	16% PEG3350, 100 mM Na citrate pH 5.4, 100mM Carboxylic acids