7ZUV

Crystal structure of Chlamydomonas reinhardtii chloroplastic sedoheptulose-1,7-bisphosphatase in reducing conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-04-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	53.774, 163.462, 172.765
Unit cell angles	90.00, 91.94, 90.00

Refinement procedure

Resolution	48.540 - 3.110
R-factor	0.1964
Rwork	0.195
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b2o
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	3.221
High resolution limit [Å]	3.110	3.110
Number of reflections	53456	5213
<I/σ(I)>	8.54
Completeness [%]	99.7
Redundancy	7
CC(1/2)	0.988	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	200mM lithium sulfate, 100mM tris-HCl pH7, 1 M sodium/potassium tartrate