7ZUU

HUMAN PRMT5:MEP50 Crystal Structure With MTA and Fragment Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	281
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9197
Spacegroup name	I 2 2 2
Unit cell lengths	103.967, 139.644, 178.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.940 - 2.090
R-factor	0.2309
Rwork	0.228
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eml
RMSD bond length	0.007
RMSD bond angle	1.433
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.940	2.360
High resolution limit [Å]	2.090	2.090
Number of reflections	39581	1971
<I/σ(I)>	9.1
Completeness [%]	93.0	71
Redundancy	8.5
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	13% PEG3350, 100 mM Na citrate, pH 5.4, 100mM Carboxylic acids