7ZUG
Heterogeneous nuclear ribonucleoprotein H1, qRRM2 domain
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.88560 |
| Spacegroup name | P 62 |
| Unit cell lengths | 81.295, 81.295, 30.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.404 - 1.075 |
| Rwork | 0.166 |
| R-free | 0.20080 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.715 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.404 | 1.090 |
| High resolution limit [Å] | 1.075 | 1.075 |
| Number of reflections | 48313 | 2420 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 96.5 | 99.8 |
| Redundancy | 9.6 | 5.8 |
| CC(1/2) | 0.999 | 0.304 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | KI 200 mM PEG 3350 20% |
