7ZU6

Polyoxidovanadate interaction with proteins: crystal structure of lysozyme bound to tetra-vanadate ion (structure 1)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	P 43 21 2
Unit cell lengths	77.050, 77.050, 37.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.520 - 1.183
Rwork	0.171
R-free	0.19610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.019
RMSD bond angle	2.095
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.520	1.200
High resolution limit [Å]	1.180	1.180
Rmerge	0.055	0.872
Number of reflections	37733	1844
<I/σ(I)>	24.1	2.3
Completeness [%]	99.9	100
Redundancy	18	8.8
CC(1/2)	0.999	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2.0 M sodium formate 0.1 M hepes buffer pH 7.5