7ZU4

Crystal Structure of Human Parechovirus 1 2A protein lacking the C-terminal oligomerisation helix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	P 43 21 2
Unit cell lengths	76.425, 76.425, 121.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.390 - 2.100
Rwork	0.198
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zu3
RMSD bond length	0.004
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.090	1.209
Rpim	0.047	0.618
Number of reflections	21802	1740
<I/σ(I)>	15.9
Completeness [%]	100.0
Redundancy	8.6
CC(1/2)	0.999	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	15% PEG 8000 0.5 M lithium sulphate