7ZU3

Crystal Structure of Human Parechovirus 1 2A protein lacking the C-terminal oligomerisation helix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	50.692, 71.666, 32.704
Unit cell angles	90.00, 97.10, 90.00

Refinement procedure

Resolution	41.173 - 1.740
Rwork	0.199
R-free	0.22570
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.173	1.770
High resolution limit [Å]	1.740	1.740
Rmerge	0.056	1.094
Rpim	0.035	0.675
Number of reflections	11948	653
<I/σ(I)>	16.3	1.7
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.998	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M Hepes-NAOH pH7.0 15% PEG 4000