7ZSV
F32V Cytochrome c prime beta from Methylococcus capsulatus (Bath) Chemically Reduced Ferrous Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-01 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.90001 |
Spacegroup name | P 21 3 |
Unit cell lengths | 105.803, 105.803, 105.803 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.960 - 1.740 |
R-factor | 0.1829 |
Rwork | 0.182 |
R-free | 0.20870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hih |
RMSD bond length | 0.016 |
RMSD bond angle | 2.163 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.100 | 61.130 | 1.790 |
High resolution limit [Å] | 1.740 | 4.770 | 1.760 |
Rmeas | 0.061 | 0.027 | 2.344 |
Rpim | 0.023 | 0.010 | 0.894 |
Total number of observations | 14289 | 13421 | |
Number of reflections | 39471 | 2114 | 2001 |
<I/σ(I)> | 19.7 | 96.2 | 0.7 |
Completeness [%] | 99.7 | 100 | 100 |
Redundancy | 6.7 | 6.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 microlitres of 15 mg/ml protein in 0.1 M HEPES buffer, pH 7.5, mixed with with an equivalent volume of reservoir solution containing 0.01 M ZnSO4, 30% PEG 550 (v/v) and 0.1 M MES, pH 6.5. Cryoprotection in ML plus 10% glycerol |