7ZSI
Structure of Orange Carotenoid Protein with canthaxanthin bound after 5 minutes of illumination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-04 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.780, 82.780, 87.604 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.542 - 1.399 |
| Rwork | 0.191 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xb5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.738 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.542 | 1.440 |
| High resolution limit [Å] | 1.399 | 1.400 |
| Rmerge | 0.133 | 9.900 |
| Rmeas | 0.140 | 11.510 |
| Rpim | 0.044 | 3.645 |
| Number of reflections | 68765 | 5049 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100 mM sodium acetate pH 4.5, 10 % poly-ethylene glycol 20,000, 3 % glycerol |
