7ZSG
Structure of Orange Carotenoid Protein with canthaxanthin bound after 1 minute of illumination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.580, 82.580, 87.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.364 - 1.390 |
| Rwork | 0.194 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xb5 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.325 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.490 | 1.390 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.049 | 2.448 |
| Rmeas | 0.051 | 2.661 |
| Rpim | 0.016 | 1.020 |
| Number of reflections | 80954 | 5917 |
| <I/σ(I)> | 23.8 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.7 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100 mM sodium acetate pH 4.5, 10 % poly-ethylene glycol 20,000, 3 % glycerol |
