7ZQZ
F61V Cytochrome c prime beta from Methylococcus capsulatus (Bath): NO complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 106.236, 106.236, 106.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.590 - 1.680 |
| R-factor | 0.1792 |
| Rwork | 0.178 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hih |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.859 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.590 | 33.590 | 1.720 |
| High resolution limit [Å] | 1.680 | 7.510 | 1.680 |
| Rmerge | 0.042 | 0.026 | 0.758 |
| Rmeas | 0.050 | 0.031 | 0.919 |
| Rpim | 0.028 | 0.017 | 0.511 |
| Total number of observations | 137210 | 1624 | 9793 |
| Number of reflections | 45336 | 550 | 3330 |
| <I/σ(I)> | 11.7 | 39.5 | 1.1 |
| Completeness [%] | 99.3 | 98.1 | 99.5 |
| Redundancy | 3 | 3 | 2.9 |
| CC(1/2) | 0.999 | 0.998 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 microlitres of 15 mg/ml protein in 0.1 M HEPES buffer, pH 7.5, mixed with with an equivalent volume of reservoir solution containing 0.01 M ZnSO4, 35% PEG 550 (v/v) and 0.1 M MES, pH 6.5. Cryoprotection in ML plus 10% glycerol |
