Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.75003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.870, 30.900, 43.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.450 - 1.410 |
| Rwork | 0.192 |
| R-free | 0.22160 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2elg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.427 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.100 | 1.440 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.118 | 0.189 |
| Rpim | 0.047 | 0.179 |
| Number of reflections | 3922 | 271 |
| <I/σ(I)> | 7.8 | 3.1 |
| Completeness [%] | 77.4 | 75.1 |
| Redundancy | 5.8 | 5.7 |
| CC(1/2) | 0.990 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.9 | 298 | 10 mg in 20 % MPD solution (cacodylate buffer + 0.01 M MgCl2) |
