7ZQJ
MHC class I from a wild bird in complex with a nonameric peptide P3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.793, 65.977, 122.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.940 - 2.250 |
R-factor | 0.19919 |
Rwork | 0.198 |
R-free | 0.23099 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gjx |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.940 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 20176 | 1819 |
<I/σ(I)> | 8.1 | 1.7 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 5.9 | 5.7 |
CC(1/2) | 0.995 | 0.418 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, pH 6.0, 6 % (v/v) Tacsimate, pH 6.0, 25 % (w/v) PEG 4000 |