7ZQ3

Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii (CrGAPA) complexed with NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	C 2 2 2
Unit cell lengths	141.984, 148.511, 74.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.740 - 1.500
R-factor	0.1877
Rwork	0.187
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rm4
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19rc7-4070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.740	46.740	1.530
High resolution limit [Å]	1.500	8.220	1.500
Rmerge	0.150	0.110	0.812
Rmeas	0.157	0.114	0.844
Rpim	0.043	0.031	0.228
Total number of observations	1629730	10774	81679
Number of reflections	124828	853	6138
<I/σ(I)>	10.6	18.7	3
Completeness [%]	100.0	99.2	99.9
Redundancy	13.1	12.6	13.3
CC(1/2)	0.995	0.989	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2-1.6 M (NH4)2SO4, 0.1 M Tris-HCl