7ZPU

Crystal structure of MreB from Geobacillus stearothermophilus ATCC7953 in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	291
Detector technology	PIXEL
Collection date	2019-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	46.220, 61.600, 50.970
Unit cell angles	90.00, 111.53, 90.00

Refinement procedure

Resolution	47.410 - 1.960
R-factor	0.1967
Rwork	0.194
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zpt
RMSD bond length	0.008
RMSD bond angle	0.983
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.420	2.080
High resolution limit [Å]	1.960	1.960
Rmeas	0.090	1.350
Number of reflections	19153	2999
<I/σ(I)>	10.56	0.86
Completeness [%]	99.4
Redundancy	6.96
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 300, 2-(N-morpholino)ethanesulfonic acid