7ZPC
CDK2 in complex 9K-DOS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.898 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.211, 71.980, 72.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.981 - 1.400 |
| Rwork | 0.201 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mhq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.815 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0340) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.981 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.022 | 0.470 |
| Number of reflections | 55242 | 2663 |
| <I/σ(I)> | 28.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.8 | 12.5 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10MG/ML CDK2 10% V/V PEG3350, 50 MM HEPES/NAOH, 50 MM NA/K PHOSPHATE, PH 7.5 |
