7ZOV

Crystal structure of Synechocystis halorhodopsin (SyHR), Cl-pumping mode, K state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 3 2 1
Unit cell lengths	61.990, 61.990, 109.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.950 - 1.700
R-factor	0.1869
Rwork	0.185
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hyj
RMSD bond length	0.006
RMSD bond angle	0.816
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.230	1.730
High resolution limit [Å]	1.700	1.700
Rpim	0.101	0.975
Number of reflections	27378	1379
<I/σ(I)>	5.5
Completeness [%]	99.2
Redundancy	10.7
CC(1/2)	0.996	0.306

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.6	293	1.6 M Ammonium Phosphate