7ZOS

Class 1 Phytoglobin from Sugar beet (BvPgb1.2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 4 21 2
Unit cell lengths	96.750, 96.750, 47.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.900 - 1.900
R-factor	0.2038
Rwork	0.203
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zhw
RMSD bond length	0.011
RMSD bond angle	1.465
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.413	1.934
High resolution limit [Å]	1.900	1.901
Number of reflections	18443	907
<I/σ(I)>	23.7
Completeness [%]	100.0
Redundancy	25.6
CC(1/2)	0.999	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20% (w/v) PEG6000, 0.1 M Citrate, pH 5.0