7ZOM
Crystal structure of N-terminal catalytic domain of human PLAAT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97632 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.638, 60.638, 74.069 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.840 - 1.601 |
| Rwork | 0.185 |
| R-free | 0.20910 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.313 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.840 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.651 |
| Rmeas | 0.039 | 0.779 |
| Rpim | 0.021 | 0.423 |
| Number of reflections | 21212 | 999 |
| <I/σ(I)> | 21.4 | 2.5 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Hepes-NaOH pH7.0-7.5, 45-70% MPD |
