7ZO6

L1 metallo-beta-lactamase in complex with hydrolysed cefoxitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97628
Spacegroup name	P 64 2 2
Unit cell lengths	105.345, 105.345, 97.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.620 - 1.610
R-factor	0.1653
Rwork	0.164
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o0o
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.740	1.640
High resolution limit [Å]	1.610	1.610
Rpim	0.016	1.058
Number of reflections	42005	2063
<I/σ(I)>	21.3	0.4
Completeness [%]	100.0	99.9
Redundancy	18.9
CC(1/2)	1.000	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM Hepes pH 7.75, 2.0 M ammonium sulphate, 1.5% PEG400. 1 ul protein (15 mg/ml) mixed with 1 ul reservoir.