7ZNX
Crystal structure of cocaprin 1, inhibitor of cysteine and aspartic proteases from Coprinopsis cinerea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-01-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.067170 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.664, 94.403, 52.151 |
| Unit cell angles | 90.00, 103.06, 90.00 |
Refinement procedure
| Resolution | 34.580 - 1.600 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iho |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.677 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.580 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 41795 | 5983 |
| <I/σ(I)> | 26.43 | |
| Completeness [%] | 97.2 | |
| Redundancy | 7.397 | |
| CC(1/2) | 0.999 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES, 1.3 M Trisodium citrate, pH=7.5 |
