7ZM5
Structure of Mossman virus receptor binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.200, 83.930, 122.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.380 - 1.620 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21160 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.170 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.080 | 1.470 |
| Rpim | 0.030 | 0.680 |
| Number of reflections | 105014 | 10324 |
| <I/σ(I)> | 13 | 1.1 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6.5 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M potassium bromide, 0.2 M potassium thiocynate, 0.1 M sodium cacodylate pH 6.5, 3% PGA-LM, 20% w/v polyethylene glycol (PEG) 550 MME |
